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</html>";s:4:"text";s:8224:"<p>Clipboard, Search History, and several other advanced features are temporarily unavailable. PLoS One. For [cAMP]pm measurements, the islets or cells were infected with cAMP biosensor-encoding adenoviruses using 105 fluorescence forming units (FFU)/islet or 60 FFU/cell as previously described (12). Ca2+ is probably not the only messenger in glucose-regulated glucagon release (29). Because Ca2+ is known to increase islet cAMP via Ca2+/calmodulin-sensitive ACs (17), it seems possible that the changes of [cAMP]pm are driven by those of [Ca2+]i (46). The interruption probably was not mediated by changes in cAMP, given that it was observed also with the inactive control compound 1,3,5(10)-estratrien-3,17-β-triol (data not shown). It has been controversial whether GLP-1 receptors are present in α-cells. 6D). However, the nature of this factor has remained elusive for many years. For liver, the degradation of glucose-6-phosphate through the glycolytic pathway is inhibited. </p> <p>D: Synchronization of the glucose-induced [cAMP]pm oscillations in two islet β-cells lacking apparent direct contact. Glucose alone also induced pronounced changes in [cAMP]pm, reminiscent of those of [Ca2+]i, with an initial lowering followed by a prominent rise. [Ca2+]i imaging with Fura-PE3 was performed at 37°C with an epifluorescence microscope setup as previously described (5). SREBP-1c itself is rapidly induced by treatment of hepatocytes with insulin, providing a pathway for insulin mediation of enhanced lipogenic gene transcription (11, 12). Synergism between calcium and bicarbonate, Glucose and glucagon stimulate the secretion of somatostatin from the perfused canine pancreas, VMAT2 Safeguards β-Cells Against Dopamine Cytotoxicity Under High-Fat Diet–Induced Stress, A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes, β-Cell–Specific Deletion of HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) Reductase Causes Overt Diabetes due to Reduction of β-Cell Mass and Impaired Insulin Secretion, ADA Standards of Medical Care in Diabetes, Institutional Subscriptions and Site Licenses, Special Podcast Series: Therapeutic Inertia, Special Podcast Series: Influenza Podcasts, http://creativecommons.org/licenses/by-nc-nd/3.0/. Another result is to provide a more integrated output for lipogenic enzyme production than would be possible by means of a single factor. However, the small molecule inhibitor KH7 that is often used to define sAC activity (20,41) showed deleterious effects on β-cell metabolism, limiting its usefulness for studies of sAC in intact cells. However, like in β-cells, 11–30 mmol/L glucose triggered [cAMP]pm oscillations in many α-cells (3 of 11 at 11 mmol/L, 13 of 28 at 20 mmol/L, and 8 of 17 at 30 mmol/L glucose) (Fig. However, adrenaline still caused elevation of [cAMP]pm in the α-cells, consistent with domination of β-adrenoceptors in this cell type (40). Since this initial characterization, cAMP … The remaining cells showed a single [cAMP]pm peak followed by return to the baseline (not shown). Recently we purified and identified a previously uncharacterized transcription factor from rat liver binding to the carbohydrate responsive element of the L-type pyruvate kinase (L-PK) gene. High concentrations of GLP-1 (>50 nmol/L) induced prompt and sustained [cAMP]pm elevation in β-cells (19 of 23) that rapidly reversed upon omission of the peptide (Fig. F: Means ± SEM for the average increases of [cAMP]pm induced by 20 mmol/L glucose alone and in combination with 10 nmol/L glucagon or 100 nmol/L GLP-1. </p> <p>Molecular scaffolds for chemical wizardry: learning nature's rules for terpene cyclases. 			 |  Glucose stimulation of insulin secretion involves uptake and metabolism of the sugar in the β-cells, closure of ATP-sensitive K+ channels, and depolarization-induced Ca2+ entry generating oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i) that trigger periodic exocytosis of secretory granules (1,2). </p> <p>Diabetes Care. Instead, it heterodimerizes with Mlx, a member of the c-myc family, to form a stable heterodimer. 5B) [Ca2+]pm. Like for insulin secretion, cAMP is believed to promote glucagon release by enhancing intracellular Ca2+ mobilization, Ca2+ influx through the plasma membrane, and mobilization of secretory granules (23,24,34,35). Figure 4A–D also shows that adrenaline abolished the glucose-induced elevation of [cAMP]pm. The [cAMP]pm-lowering effect of adrenaline on β-cells became apparent under conditions where the levels of cAMP were elevated (Fig. Under these conditions of constant hyperglycemia, the plasma insulin response is biphasic with an early burst of insulin release during the first 6 min followed by a gradually progressive increase in plasma insulin concentration. 3D). Enter multiple addresses on separate lines or separate them with commas. Studies in hepatocytes have demonstrated that increased glucose metabolism is necessary for the generation of this signal. If not otherwise stated, data were obtained with cells from at least three independent islet isolations. After rinsing with Tris buffer, the MACH 3 rabbit probe alkaline phosphatase polymer kit (Biocare Medical, Concord, CA) was used for visualization according to the manufacturer’s instructions. Please enable it to take advantage of the complete set of features! NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. 5C) and sometimes after (Fig. Lipogenesis is regulated through the acute control of key enzyme activities by means of allosteric and covalent modifications (1). The authors thank Drs. </p> <p>Through promoter-mapping studies, a glucose- or carbohydrate-response element was identified in both of these genes (14–16). Saari TJ, Raiko J, U-Din M, Niemi T, Taittonen M, Laine J, Savisto N, Haaparanta-Solin M, Nuutila P, Virtanen KA. </p> <p>2020 Sep 25;15(9):e0239506. The transmembrane adenylyl cyclase (AC) inhibitor 2′5′-dideoxyadenosine suppressed the glucose- and hormone-induced [cAMP]pm elevations, whereas the preferential inhibitors of soluble AC, KH7, and 1,3,5(10)-estratrien-2,3,17-β-triol perturbed cell metabolism and lacked effect, respectively. Some experiments were performed on single cells prepared by shaking the freshly isolated islets in a Ca2+-deficient medium followed by attachment to coverslips during 2–5 days of culture. CONCLUSIONS Oscillatory [cAMP]pm signaling in secretagogue-stimulated β-cells is maintained within intact islets and depends on transmembrane AC activity. The locations of ChREBP, NLS,…, Effects of domain deletion of ChREBP on transcription activity of the L-PK gene.…, Effects of H-89 and cantharidic acid on transcription activity of L-PK gene in…, Representative images of subcellular localization…, Representative images of subcellular localization of GFP-fused ChREBP under low or high glucose.…, (  A  ) Effect of NLS domain deletion, H-89, and cantharidic acid on…, Effect of db-cAMP on the DNA-binding activity of ChREBP  in vivo  . Emphasis in the present review has been placed on carbohydrate metabolism, but lipid metabolism has also been discussed to some extent. A method for assessment of beta-cell sensitivity and insulin resistance. At concentrations of 0.5–10 nmol/L, GLP-1 induced [cAMP]pm oscillations in β-cells (Fig. C: Dose dependence of the GLP-1 induced time-average [cAMP]pm elevation in islet β-cells. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. (First Published November 6, 2001; 10.1073/pnas.231370798), . (, Effect of db-cAMP on the DNA-binding activity of ChREBP, Schematic representation of possible regulatory mechanisms of ChREBP by glucose and cAMP in hepatocytes. 			NIH </p> <p>Farmer interviews offer insight into past motivations and future trends. Under these steady-state conditions of euglycemia, the glucose infusion rate equals glucose uptake by all the tissues in the body and is therefore a measure of tissue sensitivity to exogenous insulin. </p>";s:7:"keyword";s:12:"glucose camp";s:5:"links";s:3628:"<a href='https://crosscurrentcommunications.net/docs/b14002-principles-of-flight'>Principles Of Flight</a>,
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